Facile Detection of Mitochondrial DNA Mutations in Tumors and bodily Fluids

Makiko S. Fliss, Henning Usadel, Otavia L. Caballero, et al.
Abramson Cancer Center of the University of Pennsylvania
Ultima Vez Modificado: 1 de noviembre del 2001

Share article


Imprima English

Reviewers: Li Liu, MD
Source: Science, Volume 287(5460): 2017-2019, (March) 2000

Précis: Bodily fluid testing may detect come cancers

Introduction

Any non-invasive techniques that would aid early detection of cancers would clearly be welcomed. Some studies have demonstrated that high levels of mitochondrial DNA (mtDNA) are ubiquitous in human cells and most copies of mtDNA are identical at birth. Even slight alterations in DNA sequences could have profound effects, including cancer formation. In this study, the researchers evaluated the mtDNA in tumor samples and bodily fluids of cancer patients.

Method

A total of 14 bladder, 13 head and neck, and 14 lung cancer samples were evaluated for mtDNA mutations and the results were compared with mtDNA from patients? bodily fluids.

Results

  • 292 mtDNA sequence variations were detected in cancer samples.

  • Mutant mtDNA derived from tumor tissues was found in all urine samples obtained from patients with bladder cancer, in two third of the saliva samples from patients with head and neck cancer, and in 80% bronchoalveolar lavage samples from patients with lung cancer.

Discussion

Mutated mitochondrial DNA can be found in bodily fluids and may lead to simpler diagnosis of some cancers. Additional investigation in broader patient population is needed before it can be used as a reliable clinical test for cancer diagnosis.

Imprima English
News
Ovarian Cancer DNA Detected in Vaginal Fluid

Oct 9, 2014 - Researchers have found it's possible to detect ovarian cancer gene mutations in vaginal fluid samples -- a finding they hope is a step toward an effective screening test for the disease. The findings were published online Oct. 6 in Obstetrics & Gynecology.



I Wish You Knew

How cancer patients have changed my life

View More



Blogs and Web Chats

OncoLink Blogs give our readers a chance to react to and comment on key cancer news topics and provides a forum for OncoLink Experts and readers to share opinions and learn from each other.




OncoLink OncoPilot

Frente a un nuevo diagnóstico de cáncer o de cambiar el curso de su tratamiento actual? Deje que nuestro personal de enfermería cáncer que ayudan a pasar!

Más información